Tubular subviral structure produced in adenovirus-infected KB cells
نویسندگان
چکیده
منابع مشابه
Transport of Viral Rna in Kb Cells Infected with Adenovirus Type 2
Messenger RNA transport was studied in KB cells infected with the nuclear DNA virus adenovirus type 2. Addition of 0.04 microg/ml of actinomycin completes the inhibition of ribosome synthesis normally observed late after infection and apparently does not alter the pattern of viral RNA synthesis: Hybridization-inhibition experiments indicate that similar viral RNA sequences are transcribed in ce...
متن کاملPurification, properties, and subunit structure of deoxyribonucleic acid-dependent ribonucleic acid polymerase III from uninfected and adenovirus 2-infected KB cells.
Class III DNA-dependent RNA polymerases have been purified from human KB ceils and from KB cells infected with adenovirus 2 by chromatography on heparin-sepharose, DEAE-cellulose, DEAE-Sephadex, CM-Sephadex, and phosphocellulose and by sedimentation in sucrose density gradients. RNA polymerase III from the two cell sources has been purified approximately 14,500and 32,000-fold, respectively, rel...
متن کاملCritical role for arginine methylation in adenovirus-infected cells.
During the late stages of adenovirus infection, the 100K protein (100K) inhibits the translation of cellular messages in the cytoplasm and regulates hexon trimerization and assembly in the nucleus. However, it is not known how it switches between these two functions. Here we show that 100K is methylated on arginine residues at its C terminus during infection and that this region is necessary fo...
متن کاملPolyamines in type 5 adenovirus-infected cells and virions.
The incorporation of l-[(14)C]ornithine and [(14)C]putrescine into putrescine, spermidine, and spermine in type 5 adenovirus-infected KB cells was identical to that in uninfected control cells early in infection, but incorporation into putrescine stopped after 8 to 12 h and the rate of incorporation into spermidine was reduced between 12 and 20 h after infection. The amount of polyamines found ...
متن کاملInitiation of transcription in nuclei isolated from adenovirus infected cells
Initiation of adenovirus transcription was analyzed by incubation of isolated nuclei from virus infected cells in the presence of beta-32P GTP or beta-32P ATP. Nucleotide analysis of RNA from nuclei incubated with beta-32P GTP shows that the label is incorporated exclusively into pppGp and ppGp. Under similar incubation conditions, the label from beta-32P ATP was incorporated primarily into the...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1979
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.31.2.568-574.1979